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Dr. Ming-Daw Tsai
Distinguished Research Fellow
Room 316, Institute of Biological Chemistry, Academia Sinica
128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
TEL: +886-2-2785-5696 ext. 3070
FAX: +886-2-2788-9759
mdtsai@gate.sinica.edu.tw
Ming-Daw Tsai's Website


Currently there are two main research subjects in the Tsai lab. One focuses on the protein-phosphoprotein interaction related to DNA damage response and cancer signaling, particularly those involving phosphothreonine signaling mediated by the FHA domain. The specific systems under study include human CHK2, MDC1, Ki67, NIFK, TIFA, and yeast Rad53 kinase and Dun1 kinase. A particular emphasis is on the mechanism of activation of checkpoint kinases in vivo. The other subject is the catalytic mechanism, structure, and function of DNA polymerase involved in DNA repair. These include human DNA polymerases beta and lamda, and African swine fever virus (ASFV) DNA polymerase X. A particular emphasis is on the mechanistic differences between high-fidelity and low-fidelity DNA polymerases.

- 1978 Ph.D, Biochemistry & Medicinal Chemistry, Purdue University
- 1972 B.S., Chemistry, National Taiwan University, Taipei

2010 - present Associate Editor, Biochemistry
2008,08 - present Distinguished Research Fellow, Institute of Biological Chemistry, Academia Sinica
2012 - 2012 Academician, Academia Sinica
2008,08 - 2014,08 Director, Institute of Biological Chemistry, Academia Sinica
2006,10 - 2008,08 Acting Director, Institute of Biological Chemistry, Academia Sinica
2005 - 2008 Director, Division of Functional Genomics, Genomics Research Center
2003 - 2008 Director, National Research Program for Genomic Medicine Core Facility Office
1995 - 2003 Director, Chemistry/Biology Interface Training Program, OSU
1993 - 2007 Director, Office of Research Campus Chemical Instrument Center, The Ohio State University
1992 - 1992 Elected Fellow, American Association for the Advancement of Science (AAAS)
1992 - 1992 Distinguished Scholar Award, Ohio State University
1985 - 1990 Camille and Henry Dreyfus Teacher-Scholar
1985 - 1985 Faculty Research Award, Ohio State Chapter of Sigma Xi
1983 - 1985 Alfred P. Sloan Fellow
1981 - 2006 Kimberly Professor of Chemistry and Professor of Biochemistry, The Ohio State University (starting as Assistant Professor)

    Publications List
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation.
Wen-Jin Wu, Mei-I Su, Jian-Li Wu, Sandeep Kumar, Liang-hin Lim, Chun-Wei Eric Wang, Frank H. T. Nelissen, Ming-Chuan Chad Chen, Jurgen F. Doreleijers, Sybren S. Wijmenga, and Ming-Daw Tsai J. Am. Chem. Soc. (2014)
Use of quantitative mass spectrometric analysis to elucidate the mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo.
Eric S.-W. Chen, Nicolas C. Hoch, Shun-Chang Wang, Achille Pellicioli, Jörg Heierhorst, and Ming-Daw Tsai Mol Cell Proteomics (2014)
E339…R416 salt bridge of nucleoprotein as a feasible target for influenza virus inhibitors.
Yu-Fang Shen, Yu-Hou Chen, Shao-Ying Chu, Meng-I Lin, Hua-Ting Hsu, Pei-Yu Wu, Chao-Jung Wu, Hui-Wen Liu, Fu-Yang Lin, Gialih Lin, Pang-Hung Hsu, An-Suei Yang, Yih-Shyun E. Cheng, Ying-Ta Wu, Chi-Huey Wong*, Ming-Daw Tsai* Proceedings of the National Academy of Sciences of the United States of America (2011)
The ARID domain of the H3K4 demethylase RBP2 binds to a DNA CCGCCC motif.
Shengjiang Tu, Yu-Ching Teng, Chunhua Yuan, Ying-Ta Wu, Meng-Yu Chan, An-Ning Cheng, Po-Hsun Lin, Li-Jung Juan*, Ming-Daw Tsai* NATURE STRUCTURAL & MOLECULAR BIOLOGY (2008)
Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade.
H. Lee, C. Yuan, A. Hammet, A. Mahajan, S. W. Chen, M. R. Wu, M. I. Su, J. Heierhorst*, M. D. Tsai* Mol. Cell (2008)
Structure and Function of the Phosphothreonine-Specific FHA Domain.
Anjali Mahajan, Chunhua Yuan, Hyun Lee, Eric S.-W. Chen, Pei-Yu Wu, Ming-Daw Tsai* Science Signaling (2008)
Mechanistic comparison of high-fidelity and error-prone DNA polymerases and ligases involved in DNA repair.
Showalter AK, Lamarche BJ, Bakhtina M, Su MI, Tang KH, Tsai MD Chemical reviews (2006)
Sequential Phosphorylation and Multisite Interactions Characterize Specific Target Recognition by the FHA Domain of Ki-67.
In-Ja L. Byeon, Hongyuan Li, Haiyan Song, Angela M. Gronenborn, and Ming-Daw Tsai Nature Structural and Molecular Biology (2005)
Solution structure of a viral DNA polymerase X and evidence for a mutagenic function.
A. K. Showalter, I. J. Byeon, M. I. Su and M. D. Tsai Nat Struct Biol (2001)
Tumor suppressor p16INK4A: determination of solution structure and analyses of its interaction with cyclin-dependent kinase 4.
I. J. Byeon, J. Li, K. Ericson, T. L. Selby, A. Tevelev, H. J. Kim, P. O'Maille and M. D. Tsai Mol Cell (1998)

Updated 2014.08.25

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